Determination of the Dissociation Constant for Polyvalent Receptors Using ELISA: A Case of M13 Phages Displaying Troponin T-Specific Peptides

Phage-derived affinity peptides have become widespread thanks to their easy selection via phage display. Interactions between a target protein and its specific peptide are similar those antibodies antigens. The strength of these non-covalent complexes may be described by the dissociation constant (Kd). In this paper, protein-specific exposed on pIII present in M13 bacteriophage virion with up five copies. Therefore, one particle can bind from ligands. Here, we discuss dependences phage-displayed ligands solution using model system based troponin T (TnT) binding phages. Moreover, method calculating Kd values ELISA experiments was developed is presented. determined picomolar range.